Recent developments in male fertility evaluation, sperm cryopreservation and artificial fertilisation, and their potential application to decapod crustacean aquaculture
نویسندگان
چکیده
To maximise productivity, a better understanding of the underlying causes subfertility that lead to inferior offspring and high mortality is imperative. In decapod crustaceans, most research has focused on female reproductive performance, with little attention given male fertility. Paternal genetic contribution critical both successful embryonic post-embryonic development. Assessment sperm quality can be direct method determine in decapods. Sperm parameters such as concentration morphology have traditionally been used but these procedures are time-consuming only assess limited number cells males. Alternative diagnostic biomarkers widely humans other mammals could adapted crustaceans may more indicative fertilisation competence performance. These predictive use fluorescent cellular dyes high-throughput flow cytometry or computer-assisted microscopic analysis evaluate viability, mitochondrial function, acrosome reaction DNA fragmentation. This review examines current advanced further explores state-of-the-art cryopreservation (conventional vs. vitrification techniques) artificial crustaceans. freezing coupled decapods permits long-term storage, controlled timing selection individuals for reproduction. Collectively, tools applied commercial broodstock management improve productivity accelerate selective breeding crustacean aquaculture industry.
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ژورنال
عنوان ژورنال: Reviews in Aquaculture
سال: 2021
ISSN: ['1753-5123', '1753-5131']
DOI: https://doi.org/10.1111/raq.12627